Monthly Archives: March 2014

The optimal patch test concentration for ascaridole as a sensitizing component of tea tree oil.

Posted on March 26, 2014 by Christoffers WA, Blömeke B, Coenraads PJ, Schuttelaar ML


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The optimal patch test concentration for ascaridole as a sensitizing component of tea tree oil.

Contact Dermatitis. 2014 Mar 20;

Authors: Christoffers WA, Blömeke B, Coenraads PJ, Schuttelaar ML

Abstract
BACKGROUND: Tea tree oil is used as a natural remedy, but is also a popular ingredient in household and cosmetic products. Oxidation of tea tree oil results in degradation products, such as ascaridole, which may cause allergic contact dermatitis.
OBJECTIVES: To identify the optimal patch test concentration for ascaridole, and to investigate the relationship between a positive reaction to ascaridole and a positive reaction to oxidized tea tree oil.
PATIENTS/MATERIALS/METHODS: Three hundred and nineteen patients with eczema were patch tested with ascaridole 1%, 2%, and 5%, and 250 patients were patch tested with oxidized tea tree oil 5%. Readings were performed on D3 and D7 according to a patch test calibration protocol.
RESULTS: With an increasing ascaridole test concentration, the frequency of positive reactions increased: ascaridole 1%, 1.4%; ascaridole 2%, 5.5%; and ascaridole 5%, 7.2%. However, the frequencies of irritant and doubtful reactions also increased, especially for ascaridole 5%. A positive reaction to ascaridole was related to a positive reaction to tea tree oil.
CONCLUSIONS: This study is in support of ascaridole being a sensitizer. We recommend patch testing with ascaridole at 2%. The finding that every positive reaction to oxidized tea tree oil is accompanied by a positive reaction to ascaridole suggests that ascaridole might be a contact allergen in oxidized tea tree oil.

PMID: 24645715 [PubMed – as supplied by publisher]

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Two decades of occupational (meth)acrylate patch test results and focus on isobornyl acrylate.

Posted on March 26, 2014 by Christoffers WA, Coenraads PJ, Schuttelaar ML

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Two decades of occupational (meth)acrylate patch test results and focus on isobornyl acrylate.
Contact Dermatitis. 2013 Aug;69(2):86-92
Authors: Christoffers WA, Coenraads PJ, Schuttelaar ML
Abstract
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Plectin mutations underlie epidermolysis bullosa simplex in 8% of patients.

Posted on March 6, 2014 by Bolling MC, Jongbloed JD, Boven LG, Diercks GF, Smith FJ, McLean WH, Jonkman MF

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Plectin mutations underlie epidermolysis bullosa simplex in 8% of patients.
J Invest Dermatol. 2014 Jan;134(1):273-6
Authors: Bolling MC, Jongbloed JD, Boven LG, Diercks GF, Smith FJ, McLean WH, Jonkman MF
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Successful therapeutic transplantation of revertant skin in epidermolysis bullosa.

Posted on March 6, 2014 by Gosty?ski A, Pasmooij AM, Jonkman MF


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Successful therapeutic transplantation of revertant skin in epidermolysis bullosa.

J Am Acad Dermatol. 2014 Jan;70(1):98-101

Authors: Gostyński A, Pasmooij AM, Jonkman MF

Abstract
BACKGROUND: Epidermolysis bullosa (EB) is a group of genetic blistering diseases. Despite many efforts, treatment for EB remains symptomatic. Revertant mosaicism, coexistence of cells carrying disease-causing mutations with cells in which the inherited mutation is genetically corrected by a spontaneous genetic event (revertant cells) in 1 individual, can be found in EB. The naturally corrected revertant keratinocytes provide an opportunity for autologous cell therapy.
OBJECTIVE: We sought to locally treat EB by transplantation of revertant skin.
METHODS: Persistent ulcers in a patient with non-Herlitz junctional EB caused by mutations in the LAMB3 gene were treated by transplantation of split-thickness biopsy specimens from one of his revertant patches.
RESULTS: All transplanted biopsy specimens were accepted and complete re-epithelialization occurred within 14 days. During 18 months of follow-up, the patient never experienced blisters or wounds in the grafted area, nor in the healed donor site. Immunofluorescence and DNA sequencing showed that acceptor sites healed with transplanted revertant keratinocytes.
LIMITATIONS: Punch grafting allows only limited expansion of revertant skin.
CONCLUSIONS: We demonstrate that phenotypical and genotypical correction of skin in patients with revertant mosaicism by expansion of revertant skin might be a promising therapeutic option for cutaneous manifestations of EB.

PMID: 24176523 [PubMed – indexed for MEDLINE]

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